Abstract:In China, gas cannons have been commonly applied in the airports to repel birds, by the high decibel noise generated by the gas explosion. However, the effectiveness of bird repelling varied due to long-term exposure to the noise, or unsuitable deployment time of the Gas cannon, making birds subjective to be habituated and even insensitive to noise. Quantitative evaluation of the effectiveness of gas cannons to repel birds is still scarce. In this study, adult and healthy domestic pigeons (Columba livia domestica) were used as the research object. 40 pigeons were randomly divided into eight groups, one non-treated control group, aiming to investigate the effects of background noise on corticosterone changes, and seven interference groups with a 20 m distance interval to the noise source. Fecal samples were collected the day before noise treatment, and then the pigeons were treated with gas cannon noise continuously with a frequency of 4 times/h for 20 days, during which samples were collected at day 2, 5, 8, 11, 14, 17 and 20. Fecal corticosterone was measured using ELISA. The fecal corticosterone concentration was used as the dependent variable, and the noise duration and the distance to the noise source as independent variables. The correlation between the two variables was analyzed using Pearson correlation analysis. Difference analysis was performed using one-way analysis of variance (ANOVA). The maximum corticosterone content was 174.67 ± 5.09 ng/g at 10 m, and a minimum of 139.42 ± 0.92 ng/g appeared at 130 m (Fig. 2). There was a significant negative correlation between the distant to noise resources and the corticosterone content in the feces (Pearson′s r =﹣0.954, P < 0.01) (Fig. 1). The noise duration and the distance to the noise source have a significant interaction effect on the fecal corticosterone (F = 15.008, P < 0.01) (Table 1). As the distance increased, duration time for induced corticosterone back to the baseline showed a decreasing, and corticosterone did not change significantly with the time span beyond the noise distance of 90 m (Fig. 2). Fecal corticosterone could be an effective indicator to reflect the stress of birds to noise, and the effectiveness of gas cannons was evaluated, providing a scientific basis for control and prevention of bird strikes at the airport.