Abstract:In October 2017, some Craspedacustas were discovered in Fuquan County, Guizhou province. In order to determine its taxonomy, we first observed and measured its morphological structures,and then amplified and sequenced its 18S rRNA, ITS and COI by PCR and DNA sequencing,, then finally compared its 18S rRNA, ITS and COI gene sequences with other Craspedacustas’ gene sequences in GenBank. The results showed that the umbrella diameter, level of tentacles, shape of statocyst and other morphology structures of Craspedacusta in Fuquan were highly similar to those of Craspedacusta sowerbyi (Table 1 and Table 5); the 18S rRNA and COI sequences of Craspedacusta in Fuquan was highly similar to those of C. sowerbyi (KY077287.1, MF000493.1), and the similarity rates were 100%, 99% respectively; the ITS sequences of Craspedacusta in Fuquan was highly similar to that of C. ziguiensis (AY513637.1), and the similarity rate was 99%. The genetic distances of 18s rRNA, ITS, COI gene sequences between Craspedacusta in Fuquan and C. sowerbyi were 0.001, 0.023, 0.012 respectively (Table 2﹣4). Based on 18s rRNA and COI gene region,the Neighbor-Joining (NJ) trees showed that Craspedacusta in Fuquan was the same species with C. sowerbyi; Based on ITS gene region,the NJ trees showed that Craspedacusta in Fuquan was the same species with C. ziguiensis (Fig. 6﹣8). Taken consideration of all the results of morphological observation and molecular identification, we determined that the Craspedacusta in Fuquan, Guizhou province was C. sowerbyi. We discussed some standards of morphological structure index of Craspedacusta, the possibility of using difference between umbrella diameters as a new classification standard in morphological taxonomy and give some opinions on methods for analyzing morphological structure.