Abstract:The Tibetan Plateau Toad (Bufo tibetanus) lives in the plateau regions at 2 400﹣4 300 m above sea level. This is the first report to analyze the call characteristics and auditory sensitivity of male toad. Calls of 6 individuals were recorded by directional microphone connected to a digital audio recorder. Auditory sensitivity of 13 males was measured by auditory brainstem response (ABR). The waveform and spectrogram of advertisement calls were drawn using Praat software (Fig. 1). The acoustic properties were obtained using Adobe Audition software. The advertisement call of male toad consists of a single note with a dominant frequency of 1 150 ± 99 Hz. ABR to tone pips were characterized by valley-peak waveforms (Fig. 2). The audiogram shows that the most sensitive frequency region is 1.4﹣2.0 kHz, but the ABR thresholds are above 70 dB across 0.6﹣6.0 kHz which means that this toad has a poor auditory sensitivity compared with other species (Fig. 3). The power spectra of the male call overlap the audiogram at 1.0﹣1.4 kHz, which supported the matched filter hypothesis, although the best auditory sensitive frequency (1.6 kHz) is slightly higher than the dominant frequency of the male call.