During the fieldworks in June 2018, an adult male and two adult females (Fig. 1 and Table 1) were collected from Wenshan National Nature Reserve (23°21′41″ N, 104°50′26″ E, altitude 1 300 m) near the town of Fadou, Xichou County, Yunnan Province, China. Morphological measurements were taken for the three specimens. They have smooth olive dorsum with irregular dark-green marks and brown spots; tips of the fingers widely dilated and truncated; males with nearly entire webbed while female one third wedd; males with two side protuberant osseous tubercles on the tips of fingers while female absent; the chest of males beige with small lemon-colored spots, while the chest of female absent of spots with uniformly milk white. All major morphometric characters of these specimens were found to match with the type specimen of Kaloula nonggangensis. Phylogenetic analysis based on 16S rRNA gene fragments showed that these specimens clustered with K. nonggangensis from the type locality (Fig. 2) and shared a haplotype. Therefore, we report K. nonggangensis as a new member of amphibian fauna of Yunnan. Morphological variation and additional natural history data of this species were described. This new record found suggestion similar animal fauna were in the karst areas from southeastern Yunnan and west eastern Guangxi.