The checklist of extant, native reptiles of Zhejiang Province, China is updated based on Fauna of Zhejiang Amphibia & Reptilia in 1990 and newly published data to October 2019, along with its fauna being analyzed. In total, 89 reptile species and one subspecies are recorded in Zhejiang, belonging to 54 genera, 18 families and three orders (Table 1, Appendix). Among these reptiles, there are 77 species and one subspecies of Squamata (belonging to 43 genera and 12 families), 11 species of Testudines (belonging to 10 genera and five families) and one species of Crocodylia (in one genus and one family) (Table 2). Among them, twenty species are endemic to China; two species, five species and 16 species are listed as the national first-grade, national second-grade and provincial key protected species, respectively. Furthermore, seven species are listed by CITES appendices I; six species by CITES appendices II; two species by CITES appendices III, respectively. According to the IUCN Red List of Threatened Species (IUCN-RL), two species and six species are listed as critically endangered (CR) and endangered (EN), respectively. Ten species and 11 species are listed in the Red List of China’s Vertebrates (RLCV) as critically endangered (CR) and endangered (EN) (Appendix 1). By realm, oriental species are dominant (69 species), and the others are widespread species (18 species) and palaearctic species (two species) (Table 3). By ecotype, terrestrial species are dominant (61 species) (Table 4). The results can be used as basic data for the systematics, phylogeography and ecology of reptile. This study has an important guiding significance to protect and monitor the reptiles in Zhejiang Province, China.