Tylototriton kweichowensis is an endemic species in China and was listed in Grade Ⅱ of National protected wildlife. It has important guiding significance for environmental changes. From September 2018 to October 2019, using field observations were made on the habitats, morphological characteristics, and reproductive behavior of T. kweichowensis in the demonstration area for comprehensive management of rocky desertification in Salaxi, Bijie City, Guizhou Province. The results show that, T. kweichowensis lives in mountain ponds, springs, reservoirs, and temporary puddles. As shown in Table 1, the total length, tail length and weight of females are higher than that of males. T. kweichowensis went out after a thunderstorm on April 18. The species began to breed on April 29, and finished their breeding at the latest on August the reproductive peak was May-June. As shown in Fig. 3 and Fig. 4, the operational sex ratio of T. kweichowensis in breeding period is bias to male, but it is more female in spawn periods temprorally; female and male amplectant time ranges from a few minutes to 40 minutes. After the end of the female and male amplectant, they start to spermatophore transportation. Egg-laying behavior is carried out in 1﹣2 d after, and the eggs are produced on the bottom of the breeding ground, grass or stone. The hatching rate of eggs is 55%, the average hatching time is 8 d, and the larvae can complete metamorphosis at 130 d. These studies have shown that, the reproduction and development of T. kweichowensis are greatly affected by changes in rainfall, water volume, temperature, and the breeding habitat are relatively fixed and more susceptible to human activities. Therefore, we should pay attention to the habitats protection of T. kweichowensis in the process of rocky desertification control and ecological restoration. If necessary, we should build a stable breeding habitats manually to ensure its ecological reproduction.