Based on The Mammalian Fauna of Guizhou, historical accounts and the latest research on the taxonomy and phylogeny of animals, we provided an updated species checklist with the references of mammal species in Guizhou Province, China. As of December 31, 2019, the new mammalian inventory of Guizhou has 9 orders, 29 families, 84 genera and 153 species, including 56 of bats, 38 of rodents，24 of carnivores, 19 of insectivores, seven of ungulates, four of primates, three of hares, one of scandentia and pangolins. When compared to mammal species the records in The Mammalian Fauna of Guizhou, we added 35 species, including 5 new species: Murina shuipuensis, M. fanjingshanensis, M. rongjiangensis, M. liboensis, Apodemus nigrus, one new species records in country (M. eleryi), 14 new record species from Guizhou, and 17 species were confirmed to be distributed in Guizhou. At the same time, 23 species of animals should be removed from the Guizhou animal List due to errors in specimen identification，synonyms，or confirmed not distributed in Guizhou. 19 species are listed at the National Key Protection Species Lists, including 5 species I level and 14 species as II level. According to the IUCN Red List of Threatened Species, one mammal species is Critically endangered (CR), 5 mammal species are Endangered (EN), 5 mammal species are Vulnerable, 9 mammal species are Near Threatened. On the basis of the Red List of China’s Vertebrates, 6 mammal species are Critically endangered, 5 mammal species are Endangered (EN), 17 mammal species are Vulnerable, 39 mammal species are Near Threatened.