To promote the heath assessment and medical care of captive Bornean Orangutans (Pongo pygmaeus) and provide basic parameters for supplementing the hematological database, a total of 24 blood samples from 18 healthy orangutans were collected from 6 zoos and routine and biochemistry parameters of these samples were analyzed. The subjects were divided into different groups based on sex (14 male, 10 female) and age (7 infants, 6 subadults, 11 adults), and blood parameters of different groups were compared. The results showed that the blood routine parameters of male and female orangutans were not significantly different with each other (Table 2). The lymphocyte (LYM) density decreased with age (One Way ANOVA test, F = 5.200, P < 0.05) (Table 3). Male individuals showed higher alanine aminotransferase (ALT) density than that of female (One Way ANOVA test，F = 4.911, P < 0.05), while other parameters showed no sexual difference (One Way ANOVA test, P > 0.05) (Table 4). Total protein (TP) showed significant differences among age groups (One Way ANOVA test, F = 4.715, P < 0.05) (Table 5). There was a high glucose (GLU) content in the blood of orangutans in Chinese zoos, which should be paid attention in daily husbandry.