Exploring the controlled artificial propagation is an important way of species conservation for Chinese Sturgeon (Acipenser sinensis). To accomplish it, the artificial cultured parental population is a basic demanding. In this study, by daily observation of feeding behavior (feeding proportion, feeding frequency and daily bait ratio) and monthly measurement of growth parameters (body length, body weight, body circumference and condition factor) on 14 artificial cultured reserve parental Chinese Sturgeon (15-year-old; body length, 183﹣235 cm; body weight, 71.5﹣180.5 kg), we investigated the annual variation of feeding behavior and growth performance of reserve parental Chinese Sturgeon under natural water conditions. One-way ANOVA was used to analyze the variance between different month groups, and different sex. Post-hoc Duncan Multiple Comparison Test was used to analyze the variance between months. The results showed that, both feeding proportion and feeding frequency changed significantly between-month and exhibited an obvious inter-monthly variation feature. Similar variation disciplinarian, with no significant difference (P > 0.05), were found between male and female in most of the examination time point except feeding proportion in September (P < 0.05, Fig. 2). For feeding proportion, the highest was 86.9% ± 8.6% in April, while the lowest was 35.4% ± 28.5% in July (Fig. 2). For feeding frequency, the highest was 3.01 ± 1.35 times in March, while the lowest was 0.70 ± 0.63 times in July (Fig. 3). The daily bait rates of both compound bait and fresh bait present down first and then up trend. For compound bait, the lowest daily bait ratio was 0.02% in October, while the highest was 0.19% in January. And for fresh bait, the lowest daily bait ratio was 0.12% in June, while the highest was 0.99% in April (Fig. 4). The average annual feeding proportion of fresh food was 62.57% ± 15.63%, but the compound food was only 18.39% + 17.61%, which indicated an obvious preference of fresh food in Chinese Sturgeon. Negative correlations were obtained between compound food daily bait rate, fresh food daily bait rate, total feeding proportion, female feeding proportion, total feed frequency, female feed frequency and water temperature by Pearson correlation analysis (Table 1). The parental Chinese Sturgeon displayed a slower-growing feature, and the body circumference, body weight and condition factor showed down first and then up trend. Except condition factor, no significant difference can be detected in other growth parameters during the experimental period (Table 2). Although few inadequacies may occurred in experimental conditions and sampling process, such as the square fish pond with shallow water may cause spatial stress effect, monthly inspection and sampling may cause sampling interference, the results obtained in the present study still can be referred for improving cultivation techniques of Chinese Sturgeon. To improve feeding and growth of Chinese Sturgeon, water temperature regulation, suitable breeding facility, reducing interference and optimizing feed composition should be provided during the feeding management.