We examined predation on livestock of wolf(Canis lupus)by both field survey and resident interview method in Dalai Lake Nature Reserve,Inner-Mongolia,China,from Jul 2004 to Jan 2007.Totally 95 predation were claimed by the residents,in which total of 425 livestock were killed.The residents lost 186 575.00 RMB at least from those predations.We record the date of killing,species and number of killing,age of victim,location of killing.We analyzed 9 variables by GIS technique and tested the significance of difference between those variables by Mann-Whitney U test.PCA method was employed to identify the principal components among the 9 variables.Results showed that sheep,cattle and horses were the main species of livestock that wolf preyed on,94.16% of the victims were sheep Camel and donkey were casually predated.Most predation occurred during the frozen season(Sep-Feb,89.74%).Location of predation was influence in the order of importance by human disturbance,habitat type,livestock species,distance to rest site,sheltering class,distance to water source,season,number of killed,distance to stockade.Result of principal components analysis(PCA)showed that the first 3 principal components explained 81.544% of the total variance among all predation habitat variables.According to absolute value of coefficient,the 3 components were classified separately as sheltering class factor,disturbance factor and water factor.Sheltering class factor is the chief factor influencing the location of predation.Shortage of prey is key reason of killing on livestock.