To clarify the genetic character of Tupaia belangeri from Luquan District,Kunming,we examined DNA sequence variations in the mitochondrial cytochrome b gene gotten from 30 individuals.We detected variation at 32 nucleotide sites(2.81% in the full sequences),the rate of transitions and transversions is 10.4.T.belangeri was a valid taxonomic species rather than a sub-species of T.glis based on Cyt b gene sequences;the relationship between order Scandentia and Dermoptera was closer.