国家自然科学基金项目(No.30660127);广西科学基金项目(No.桂科青0640002,0447004)
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建立了猪(Susscrofa)卵母细胞蛋白质双向电泳平台,并对裂解液的组成、样品处理、双向电泳程序等相关技术进行优化,得到清晰的微量卵母细胞蛋白质的电泳图谱。利用上述优化后的体系分别对未成熟和成熟的猪卵母细胞进行双向电泳分析,并用ImageMaser软件对图谱进行比对分析。结果表明,电泳图谱上大约有800个左右的蛋白点,其中差异蛋白35个,包括上调蛋白22个及下调蛋白13个。说明基于双向电泳的蛋白质组学可以用于卵母细胞成熟的蛋白表达差异的研究。
The two-dimensional electrophoresis(2-DE) technique for Porcine(Sus scrofa) oocyte proteome analysis was established.The components of protein lysis solution,sample treatment,and 2-DE procedure were optimized.A profile of proteins extracted from Porcine oocytes was obtained clearly.The protein expression profiles of immature and in vitro matured Porcine oocytes were compared. The ImageMaster 2-DE software was used to characterize different proteins.About 800 protein spots were identified,of which 22 proteins were up-regulated and 13 proteins were down-regulated during oocyte maturation in vitro.It proves that proteomics technique base on two-dimensional electrophoresis can be used in further studies on differential protein expressions in Porcine oocyte development.
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胡惠忠,付强,卢晟盛,张明,陆阳清,蒙冰,卢克焕.2008.猪卵母细胞蛋白质组双向电泳体系的建立及初步分析.动物学杂志,43(4):67-71.
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- 收稿日期:2007-10-28
- 最后修改日期:2008-04-30
