安徽省教育厅自然科学基金项目(No.2006kj362b);安徽医科大学引进人才基金项目(2004年)
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应用细胞内记录技术,观察并分析了银杏内酯B(GB)对豚鼠(Cavia porcellus)离体腹腔神经节(CG)神经元快兴奋性突触后电位(fast-EPSP)的影响及可能机制。用4×10-6mol/L的GB灌流CG,fast-EPSP的幅值均明显降低,与对照组相比有显著性差异(n=12,P<0.05);用低Ca2+/高Mg2+Krebs液灌流,fast-EPSP被完全抑制(n=3);用高Ca2+Krebs液灌流,fast-EPSP幅度则增大(n=12,P<0.05),而用4×10-6mol/L GB与高Ca2+Krebs液联合灌流,fast-EPSP幅度则减小(n=12,P<0.05)。结果提示,GB对CG神经元fast-EPSP的抑制效应可能与减少或抑制CG神经元的外Ca2+内流有关。
The present study was carried out to determine the regulation of ginkgolide B(GB)on fast-excitatory postsynaptic potential(fast-EPSP)in the neurons of celiac ganglion(CG)and the mechanisms involved in the GB-induced responses.Intracellular recording was employed to measure the fast-EPSP in the neurons of CG freshly isolated from Guinea Pigs(Cavia porcellus).We found that perfusion of GB(4×10-6 mol/L)significantly decreased the amplitude of fast-EPSP in the CG neurons(n=12,P<0.05).The fast-EPSP was completely inhibited by a low Ca 2+/high Mg 2+ Krebs solution(n=3),but significantly enhanced by a high Ca 2+ Krebs solution(n=12,P<0.05),suggesting a contribution of extracellular Ca 2+ to the generation of the fast-EPSP.The high Ca 2+ Krebs solution-induced augmentation of the fast-EPSP was significantly attenuated by GB(n=12,P<0.05).These results indicate that GB negatively regulates fast-EPSP in CG neurons in Guinea pigs through an inhibition on external Ca 2+ entry.
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胡金兰,孔淼,许奇,王烈成,柯道平,马嵘,孔德虎.2008.银杏内酯B对豚鼠腹腔神经节神经元Fast-EPSP的抑制效应.动物学杂志,43(5):31-36.
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- 收稿日期:2008-03-10
- 最后修改日期:2008-06-30
