The peripheral blood cells of Paracanthobrama guichenoti were morphologically to perceive as erythrocyte,neutrophil,monocyte,large or small lymphocyte and thrombocyte distinctly on the stained smears of peripheral blood,but eosinophilic granulocyte and basophilic granulocyte were not found.In addition,some immature and diving erythrocyte could be observed.Among these leucocytes,the thrombocyte was the smallest cell while neutrophil the largest;and the number of large lymphocyte was the least,while that of thrombocyte the most.The results of blood biochemical parameters assay indicated that alanine aminotransferase(ALT),cholesterol(CHOL)and total protein(TP)have a fargoing change,especially aspartate aminotransferase(AST).This study also demonstrated that male P.guichenoti resulted in a significantly lower level of relative blood biochemical parameters as compared to the female(P<0.05).