The present study dealt with the hematological and serum biochemical indices of adult Ovate Pompano Trachinotus ovatus reared in submersible cage and the relation between genders and haematological indices. The results showed that there was no significant difference on the erythrocyte number (RBC), leukocyte number (WBC), hematocrit (Ht) and erythrocyte sedimentation rate (ESR) between adult male and female. In terms of the serum biochemical indices, concentration of blood glucose was much higher in male than in female. Moreover, the concentration of low-density lipoprotein cholesterin (LDL-C), kalium (K⁺), aspartate aminotransferase (AST), alanine amiotransferase (ALT) in serum of female was higher than that of male (P<0.05). Meanwhile,the concentration of creatinine (CREA), natrium (Na⁺) in serum of male was higher than that of female. However, there was no significant difference between male and female in uric acid (UA), blood urea nitrogen (BUN), triglyceride (TG), total cholesterin (TC), high-density lipoprotein cholesterin (HDL-C), γ-glutamyl transpeptadase (γ-GT),total protein (TP), albumin (ALB), globulin (GLB), ALB/GLB, calcium (Ca²⁺), chlorine (Cl^-) and phosphorus (PO₄^3-). Some serum biochemical indices differences between adult male and female ovate pompano might be related to the physical condition of each individual. The results would be used as the normal physiological indices for Ovate Pompano.