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广西巴马小型猪克隆胚的构建及胚胎移植
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国家自然科学基金项目(No.30660127);广西科学基金项目(桂科青0640002);广西亚热带生物资源保护利用重点实验室-省部共建国家重点实验室培育基地开放课题(SB0701)


Construction and Transfer of Guangxi Bama Mini-pig Somatic Cell Nuclear Transfer Embryos
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    摘要:

    通过胚胎移植验证构建的广西巴马小型猪克隆胚是否可以发育到期。利用刺入式手术胚胎移植法,将0.5～1.5日龄巴马小型猪克隆胚移植到2头巴马小型猪和2头陆川猪的输卵管壶腹部。其中2头巴马小型猪和1头陆川猪返情,另外一头陆川猪于2007年10月13日产下1头克隆雄性巴马小型猪。说明巴马小型猪克隆胚能够在受体猪体内发育到期并产仔。

    Abstract:

    The objective of this study was to examine if Guangxi Bama Mini-pig somatic cell nuclear transfer (SCNT) embryos can develop to term after embryo transfer.Bama Mini-pig cloned embryos of 0.5-1.5 days old were surgically transferred to the oviductal ampulla of two female Bama Mini-pigs and two female Guangxi Luchuan pigs,of which the two female mini-pigs and one Luchuan pig returned to estrus,and one female Luchuan pig gave birth of one male cloned Bama mini-piglet.It is concluded that Guangxi Bama Mini-pig SCNT embryos can develop to term after embryo transfer.

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卢晟盛,吕培茹,刘红波,何若钢,潘天彪,罗龙兴,黄敏瑞,卢克焕.2008.广西巴马小型猪克隆胚的构建及胚胎移植.动物学杂志,43(6):147-153.

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  • 收稿日期:2008-04-15
  • 最后修改日期:2008-09-02
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