During the investigation into parasitic pathogens of fishes in urban areas of Urumqi and Bachu County, Xinjiang, from September 2018 to October 2019, monogenean parasites were found in the gills of both Ictalurus punctatus and I. nebulosus. The morphology of the parasitic species was basically the same between the two fish species, except for the bar morphology. The parasites were tentatively identified as Ligictaluridus pricei of Ancyrocephalidae, Ligictaluridus (Fig. 1, 2). To verify the the species identified by morphology, molecular identification was performed with the construction of a phylogenetic tree. The 28S rDNA sequences of parasites from the two fish species were amplified, and the phylogenetic tree and genetic distance matrix were constructed after sequence comparison. The similarity between the parasites from the two species and L. pricei was above 98%, and the phylogenetic tree showed that they closely clustered into a same branch with a confidence of 100 (Fig. 4, 5). The same genetic distance between the parasite from the two species and L. pricei was close to zero, showing a high degree of similarity and kinship (Table 3). Therefore, according to the morphological characteristics and phylogenetic analysis, it was determined that these two fishes were infected with the same parasite. The findings of the study can provide a basis for the disease control of artificially cultured catfish in Xinjiang region.