The mitochondrial DNA(mtDNA) has been used as a genetic marker in Gallus gallus and Anser anser,but not in the Anas platyrhychos.In this study,we designed 15 primers by referring to close species.Using PCR amplification,sequencing and assembling,we obtained the complete mitochondrial genome of Beijing Duck(A.platyrhychos).The entire mitochondrial genome was 16 604 bp in length.It contained 37 genes(13 protein coding genes,2 rRNA,and 22 tRNA) and a non-coding control region(D-loop).The composition of the nucleotides is 29.19%A,22.20%T,15.80%G,and 32.81%C.The genome organization including gene order resembled to that of other birds.Based on the sequence of D-loop region,the neighbor-joining method was adopted to examine the phylogenetic relationships among 7 Anseriform birds.The results indicated that Beijing duck and Mallard(A.platyrhychos) had closer phylogenetic relationship.