To establish tetracycline-controlled A30P mutation α-synuclein transgenic mice,pTRE2-syn and pBC-rtTA dual transgenic mice were generated by pro-nuclear microinjection and embryo transplantation.Positive founder mice were identified through PCR.An rtTA and A30P dual positive transgenic founder and thirteen A30P single transgenic founders were obtained.The expression of A30P mRNA was detected by RT-PCR in the brain of the dual transgenic mice after administration of tetracycline.In addition,the expression of α-synuclein protein in the brainstem was also significantly increased in double-transgenic mice after 4-week continuous treatment with doxycycline.