版纳鱼螈外周血细胞观察
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广西科学基金项目(No.桂科自0832285);广西教育厅科研项目(No.200504219)


Peripheral Hematocytes in Caecilian Ichthyophis bannanica
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    摘要:

    以濒危两栖动物版纳鱼螈(Ichthyophis bannanica)为材料,应用瑞氏-姬姆萨混合染色法与血细胞计数法观察并统计了版纳鱼螈各种外周血细胞的形态特征和数量比例。结果表明,版纳鱼螈的外周血液中红细胞数量较多,呈卵圆形、椭圆形、梭形和梨形,平均含量为2.57×105个/mm3。白细胞数量较少,多呈近圆形,平均含量为0.72×103个/mm3。白细胞中,淋巴细胞最多,其次为单核细胞、嗜中性粒细胞、嗜碱性粒细胞和嗜酸性粒细胞。血栓细胞数量较少,常数个集合在一起。同时,将此研究结果与鱼类、爬行类和其他两栖类的血细胞比较,进而探讨了版纳鱼螈的进化地位。

    Abstract:

    The present study analyzed morphology of the peripheral blood of Ichthyophis bannanica by the Wright-Giemsa staining.The peripheral blood of I.bannanica contained erythrocytes,thrombocytes,lymphocytes,monocytes,neutrophils,basophils and eosinophils.Erythrocytes were oval,elliptical,spindle-or pear-like cells,and their mean density was 2.57×105 cells/mm3.Most leucocytes were round,and their mean density was 0.72×103 cells/mm3.The lymphocytes were most rich leucocytes,followed by monocytes,neutrophils,basophils and eosinophils.Thrombocytes were less,and frequently appeared in groups.Meanwhile,we compared the peripheral blood of I.bannanica with that of fishes,reptiles or other amphibians.The results are discussed on the basis of the possible phylogenetic position of I.bannanica.

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李桂芬,蒙绍权,李涛,姚锦仙.2009.版纳鱼螈外周血细胞观察.动物学杂志,44(2):102-107.

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  • 收稿日期:2008-09-24
  • 最后修改日期:2009-01-23
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