The genetic polymorphism of 16 microsatellite loci in three Sika Deer(Cervus nippon)populations in Heilongjiang Province(Xingkaihu farm in Mishan region,Yinlang livestock farm in Daqing region,and Daqing farm in Wudalianchi region)was determined.Number of alleles,effective number of alleles(Ne),mean heterozygosity(h)and polymorphism information content(PIC)were calculated for each population.The results showed that 11 of 16 microsatellite loci were polymorphic except for T501,C14,C217,T107 and C273.Among the 11 loci with polymorphism,five were highly polymorphic and four were medium polymorphic,showing that these microsatellite loci are effective markers for the genetic analysis of C.nippon populations.The average heterozygosity of the three groups changed from 0.453 to 0.636 and Xingkaihu Group had the highest h(0.636),which could have more potential for selection.