A compound staining method composed of the Ponceau S picric acid,Resorein basic fuchsin and Perls stainings was used to show the distribution of collagen,elastic and muscular fibers and iron in the blood vessel.It was found that the compound staining displayed the red collagen,brown elastic and yellow muscular fibers,as well as green iron.The compound staining method could clearly identify different fibers in the blood vessels.