This paper analyzed the annuli characteristics of sagitta,part of dorsal fin ray(n=11 and 12),opercular (n=20 and 25) and vertebra(n=38 and 45) of 174 Triplophysa teunis samples and 258 T.yarkandensis samples collected from Kezi River in southern Xinjiang in May 2008,and assessed their usage in age determination.The results showed that dorsal fin ray and opercular were unsuitable for ageing,mostly because of the pseudoannuli and ambiguous annuli.The annuli characteristics on vertebra of T.teunis with body length less than 6.0 cm could not be seen easily,their interpretability and age agreement were 52.63% and 70.00% respectively.The sectioned vertebra interpretability and age agreement of T.yarkandensis were 93.33% and 78.57% respectively,but there were pseudoannuli disturbances on the sectioned vertebra of the specimen under 5 years,which will decrease the accuracy of aging.Growth markers such as daily growth increments,checks and annuli can be seen on the sectioned sagitta.The annuli characteristics of the sectioned sagitta displayed a pattern of alternating zones of widely-and closely-spaced circular,and the outer edges of the circular were annuli.The ages of 171 samples can be identified from 174 T.teunis samples,the interpretability and age agreement were 98.28% and 94.74% respectively. The ages of 244 samples can be identified from 258 T.yarkandensis samples,the interpretability and age agreement were 94.57% and 93.44% respectively.As for as ages assigned based on sectioned vertebra and sagitta from the same specimen by two independent identifiers,age agreement of T.teunis were 75.00% and 80.00% respectively,those of T.yarkandensis were 73.81% and 83.33% respectively,no significant difference between identifier 1 and 2 (P>0.05).The results indicated that sectioned sagittae were more suitable to age determination for two esquamate Triplophysa,sectioned vertebrae can be used as accessory ageing materials for older individuals.