We sampled invertebrates,water,and soil at Shanlanqiao Cave,Anshun County,Guizhou Province in June 2009. We identified 25 species belonging to 12 families,7 orders,4 classes and 2 phyla from 637 samples and grouped them into spiral shells,millipedes,Opiliones,Diestrammena marmorata,Orthomorpha 5 groups. We measured the content of 8 heavy metals Pb,Cd,Hg,As,Cu,Zn,Cr,Ni in the five animal groups and in the water and soil samples by atomic absorption spectrometer and atomic fluorescence spectrometer. The content of Zn was highest among 8 metals,in water (112. 90 μg /L),soil (224. 60 mg / kg), spiral shells ( 146. 80 mg /kg ), millipedes ( 252. 20 mg /kg ), Opiliones ( 556. 80 mg /kg ), Diestrammena marmorata ( 233. 30 mg /kg) and Orthomorpha ( 722. 50 mg /kg). Animal samples contained higher Cd,Cu and Zn than the soil samples did with an accumulation index > 1; while the accumulation index of As,Cu,Zn,Ni was > 1 000 in the animal samples copared with water samples. The same animal group had different accumulation index on different heavy metals,and different groups had different accumulation index on same heavy metal. The content and enrichment of heavy metal in animal samples might be related with theenvironment and with the relation between elements of heavy metal.