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罗非鱼mtDNA D-loop区部分序列结构和种群遗传多样性分析
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中央级公益性科研院所基本科研业务费专项资金(No.2007JBFA02);国家科技支撑计划专题(No.2006BAD01A1202);2007年公益性行业(农业)科研专项(No.3-49);现代农业产业技术体系建设专项资金(No.nycytx-48);农业部水生生物遗传育种和养殖生物学重点开放实验室开放基金课题(No.BM2007-14);国家重点基础研究发展计划973计划项目(No.2004CB117401)


Structure of the Mitochondrial DNA D-loop Region and Analysis of Genetic Diversity in Different Strains of Tilapia (Oreochromis)
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    摘要:

    采用PCR扩增和测序的方法获得了尼罗罗非鱼(Oreochromis niloticus)埃及品系(简称AJ,22尾)、88品系(简称XH,26尾)和奥利亚罗非鱼(O.aureus)(简称ALY,28尾)"夏奥1号"线粒体DNA控制区(mtDNA D-loop)的部分序列(575～581 bp)。对照其他已报道的鱼类控制区结构,对序列结构进行分析,成功识别了罗非鱼mtDNA D-loop区序列的中央保守区和保守序列区,找到了4个特征序列(CSB-D、CSB1、CSB2、CSB3)。76个个体共检测出28个单倍型。AJ群体有12个单倍型,XH群体有10个单倍型,ALY群体存在9个单倍型。其中,3个群体共享1个单倍型(XH06),AJ和XH群体共享一个单倍型(XH10),其他为各个群体独有。AJ和XH(Nm=0.77)、AJ和ALY(Nm=0.02)、XH和ALY(Nm=0.02)之间均存在一定的遗传分化。AJ和XH的核苷酸多态性(Psub>i)值(分别为0.042 4、0.031 1)明显高于ALY的Psub>i值(0.001 2)。本实验中的ALY品种纯度高,AJ和XH的遗传多样性较丰富。mtDNA D-loop区能反映出罗非鱼群体的种质特征,适用于罗非鱼群体内的遗传多样性研究。

    Abstract:

    The partial mitochondrial DNA D-Loop sequence was amplified in two populations(Egyptian and 88 strain) of Nile tilapia(Oreochromis niloticus)(AJ and XH) and Blue tilapia(‘Xia′ao No.1',O.aureus)(ALY) by PCR technique.The length of this region was 575-581 bp.Compared with its homology,one conserved block(CSB-D) in the central conserved sequence block domain(CSB) and three conserved sequence blocks(CSB1,CSB2,CSB3) in the conserved sequence block domain(CSB) were successfullyidentified.A total of 28 haplotypes were detected in 76 individualities.The number of haplotypes was 12 in the AJ population,10 in the XH population and 9 in the ALY population.Only one haplotype(XH06) was shared by three populations,and one haplotype(XH10) was shared by AJ and XH populations.Genetic diversity analysis indicated some genetic differentiations between AJ and XH(Nm=0.77),AJ and ALY(Nm=0.02),as well as XH and ALY(Nm=0.02).The nucleotide diversity(Psub>i) of AJ(0.042 4) or XH(0.031 1) was higher than that of ALY(0.001 2).It could be concluded that the purity of ALY was much higher,and that AJ and XH had a high level genetic diversity.The mtDNA D-Loop region could reflect the genetic resource characteristics and could be used to study genetic diversity of tilapia populations.

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吴长敬,邹芝英,杨弘,李大宇,祝璟琳,肖炜.2010.罗非鱼mtDNA D-loop区部分序列结构和种群遗传多样性分析.动物学杂志,45(5):121-128.

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  • 收稿日期:2010-02-22
  • 最后修改日期:2010-04-27
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