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微卫星标记分析白斑狗鱼遗传多样性
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新疆少数民族特培项目(No.200923110);国家农业成果转化资金项目(No.200654118-15-1)


Analysis of Genetic Diversity by Microsatellite Markers in Northern Pike (Esox lucius)
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    摘要:

    为了解我国境内白斑狗鱼(Esox lucius)野生群体的遗传多样性现状,利用8对微卫星引物对额尔齐斯河流域185河段、635河段、乌伦古湖及吉力湖4个地理群体的遗传多样性进行了研究。结果显示,8个微卫星位点的平均等位基因数为6.625 0,多态信息含量为0.603 6～0.656 5,可有效用于白斑狗鱼遗传多样性和遗传结构分析。4个群体的平均期望杂合度为0.712 6～0.660 0,表明我国境内野生白斑狗鱼群体有较高的遗传多样性水平。整个群体的总近交系数为0.266 6,少数个体存在近交现象,白斑狗鱼群体有近交倾向。平均分化系数为0.062 2,群体间的遗传变异占总群体变异的6.22%,白斑狗鱼各群体间的分化程度不大。群体间的基因流值变化范围为10.077 5～3.360 6,说明白斑狗鱼不同群体间存在较为广泛的基因交流。

    Abstract:

    To investigate the genetic diversity of Esox lucius in China,8 pairs of microsatellite primers were used to analyze 4 geographical populations(185 reach of Eerqisi River,635 reach of Eerqisi River,Wulungu Lake and Jili Lake) of E.lucius The results show that the average number of allele is 6.625 0 and the polymorphic information content varies between 0.603 6 and 0.656 5,which can be used for the analysis of genetic diversity and genetic structure effectively.The average expected heterozygosity values of four populations range from 0.712 6 to 0.660 0,which indicates a higher level of genetic diversity in wild populations.The total inbreeding coefficient of entire populations is 0.266 6 and the inbreeding phenomenon exists in a small number of individuals,indicating that there is a tendency toward inbreeding in E.lucius populations.The average differentiation coefficient is 0.062 2,the genetic variation among populations accounts for 6.22% of the total variation,the differentiation among populations is in a low degree and the values of gene flow among populations range from 10.077 5 to 3.360 6,indicating a wide gene exchange among E.lucius populations.

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海萨,杜劲松,孟玮,杨天燕.2010.微卫星标记分析白斑狗鱼遗传多样性.动物学杂志,45(6):106-112.

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  • 收稿日期:2010-04-30
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