国家自然科学基金项目(No.30860215)
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以肾细胞作材料,采用秋水仙素-低渗-空气干燥法、Ag-NORs、C-带和G-带显带技术对准噶尔雅罗鱼(Leuciscus merzbacheri)染色体进行了研究。结果表明:(1)准噶尔雅罗鱼2n=50,核型组成为18m+14sm+6st+12t,NF=82,没有异型性染色体分化。(2)Ag-NORs的数目在不同的细胞中表现出多态性,数目为1～2个,出现1个Ag-NORs的频率最低(10%),出现2个的频率最高(70%);Ag-NORs主要出现在m1对和m4对同源染色体上;未发现有Ag-NORs联合的现象。(3)准噶尔雅罗鱼的染色体均呈现C-带阳性,可分为着丝粒C-带和端粒C-带。(4)同源染色体上G-带带纹基本一致,其带纹在每对染色体上的数目及分布具有明显特征性。
The chromosome spreading of metaphase kidney cells was conducted by colchicine-low concentration-air drying technique in Leuciscus merzbacheri,and Ag-NORs,C-band and G-band were analyzed in this paper.L.merzbacheri had a diploid chromosome number of 2n=50,its karyotype formula was 18m + 14sm + 6st + 12t,NF=82,and sex chromosome was not found.The Ag-NORs polymorphisms were individually specific,1-2 in number,and the frequency of single Ag-NORs was the lowest(10%),while the frequency of 2 Ag-NORs was the highest(70%).Ag-NORs mostly appeared in the chromosome m1 and m4.The associations of Ag-NORs were not observed.All the chromosomes were C-band positive,and composed of centromeric C-band and telocentric C-band.The G-bands in homologous chromosomes were basically coincident,and distinctive character of banding number and distribution could be identified in every homologous chromosome.
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王佳君,胡文革,孔磊.2010.准噶尔雅罗鱼染色体核型及带型的初步研究.动物学杂志,45(6):120-126.
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- 收稿日期:2010-05-24
- 最后修改日期:2010-09-20
