Dajinshan Island is the highest and largest bedrock island in Shanghai and located on the northeast corner of Hangzhou Bay. Due to less human interference, the vegetation maintains a better status and retains the original background of precious animal and plant resources in Shanghai. We surveyed the diversity of amphibians and reptiles located in Dajinshan Island from May 2017 to October 2018. In total, 2 species of amphibians belonging to 2 families, respectively, and 8 species of reptiles belonging to 3 families were detected in this survey. We found that the reptile diversity in Dajinshan island is also the highest in Shanghai. Compared with previous survey results, the composition of amphibians and reptiles has changed. Gekko japonicus, Pelophylax plancyi, Takydromus septentrionalis and Oocatochus rufodorsatus distributed on the island have now disappeared, and Bufo gargarizans, Ptyas dhumnades, Calamaria septentrionalis and Lycodon rufozonatum are newly recorded in this survey.