The male Redclaw Crayfish (Cherax quadricarinatus) with initial body length of 10.5 cm were fed with artificial diets containing different levels of cholesterol (0,0.25%,0.50%, 0.75%,1.00%) for 10 weeks.Hepatopancreas digestive enzymes and testis AKP,LDH,ATPase were measured.The results showed that group 2 had the best growth index and the highest trypsin activity.There was no significant difference in lipase activity among groups 2,3,and 4,but lipase activities in these three groups were higher than those in group 1 and control.Testis AKP activity and LDH activity were the highest in group 3.There was no significant difference in testis ATPase activity between group 2 and 3,but ATP activities in these two groups were higher than those in groups 1,4 and control.The results suggest that the optimum dietary cholesterol in the feed was between 0.50% to 0.75% during rapid gonadal development phase in C.quadricarinatus.