Eggs of Eremias brenchleyi were incubated at three constant (27, 30 and 33℃) and one naturally fluctuating temperatures to assess the influence of incubation temperature on hatching success, hatchling traits and locomotor performance. The duration of incubation and sprint speed differed considerably among temperature treatments, whereas hatching success did not. Hatchling incubated at 33℃ were smaller in SVL and lighter in body mass and carcass dry mass but lighter in residual yolk dry mass than those at 27,30℃ and fluctuating temperatures. Similarly, the sprint speed of the hatchlings from 33℃ were also smaller than those from 27,30℃ and fluctuating temperatures. Eggs incubated at the fluctuating temperatures may experience extreme temperatures for brief periods, however, these temperatures does not have detectable adverse effects on hatching success, hatchlings phenotypic traits and sprint speed in E. brenchleyi.