Fourteen bats were captured at the Tongan Cave in Yongzhou of Hunan Province and identified as Myotis ricketti which is a new record of the Chiroptera in Hunan Province. The morphological measurements and skull characteristics were reported and compared to the same specimens in Zhejiang and Anhui Province. The species produces short echolocation calls with downward frequency-modulated(FM) components and low peak frequency. The special echolocation calls and morphology such as strong hind foot and sharp claws contribute M.ricketti to trawl fish over the water surface.