中央级公益性科研院所基本科研业务费专项资金(No.2011JBFC03, 2009JBFB05),农业部淡水生物多样性保护与利用重点开放实验室第七期开放课题(No.LFBU0706),公益性行业(农业)科研专项经费项目(No.200903046-02);
- 摘要 | |
- 访问统计 |
- 参考文献 [22] |
- 相似文献 [20] |
- 引证文献 | |
- 文章评论
实验分离了吉富罗非鱼 ( Oreochromis niloticus )两种生长激素受体基因 GHR 1和 GHR 2的全长cDNA以及 GHR1 阅读框中外显子2~9、 GHR 2阅读框外显子2~8的DNA序列。在 GHR 1和 GHR 2分别找到6个和16个SNPs位点,其中只有2个位点在外显子部分。使用PCR-RFLP方法检测了5个家系共120尾吉富罗非鱼在8个SNPs ( GHR 1内含子3_A612G、内含子3_A989T、内含子7_A599C; GHR 2内含子3_C330T、内含子3_A645G、内含子3_G687T、内含子3_A967G、内含子7_C107T)的基因型。不同基因型与雌、雄吉富罗非鱼增重的相关性分析显示, GHR 1内含子3_A612G及内含子3_A989T与雄鱼增重极显著 ( P <0.01)和显著 ( P <0.05)相关,杂合型个体增重最大,在雌鱼群体中有类似趋势,但差异不显著;内含子7_A599C与雄鱼、雌鱼增重分别呈现极显著 ( P <0.01)和显著 ( P <0.05)相关,CC型雌雄鱼增重均大于AA型; GHR 2上只有内含子3_G687T与雌鱼增重显著相关( P <0.05),GT型个体增重显著大于GG型。检测了60个家系共356尾吉富罗非鱼在3_A612G、3_A989T、7_A599C和3_G687T的基因型与增重性状的相关性,结果具有相同趋势,因样本少、遗传背景多样,导致差异性显著降低。实时定量PCR结果显示, GHR 1在雄鱼肝的表达量是雌鱼的1.36倍;除肾外, GHR 2在雄鱼各组织的表达量均低于雌鱼,这部分解释了 GHR 1和 GHR 2基因上SNP位点对雌、雄吉富罗非鱼增重的不同影响。此外,GHR1、GHR2对生长影响程度的不同,导致只在 GHR 2上找到1个SNP位点对增重有影响。
Growth hormone receptors (GHR) which located on the cell membrane is the only pathway for growth hormone (GH) to get into cells and affect the growth in animals, they play a vital important role on animal growth. Two cDNA sequences and DNA sequences of exon 2-9 of GHR 1, exon 2-8 of GHR 2 were isolated from GIFT strain (Genetically Improved Farmed Tilapia strain, Oreochromis niloticus ) using RT-PCR and PCR in this study. Six and sixteen SNPs were identified on GHR 1 and GHR 2 respectively, only two of which were located at exons. Genotypes were test in eight SNPs ( GHR 1 intron 3_A612G, intron 3_A989T, intron 7_A599C; GHR 2 intron 3_C330T, intron 3_A645G, intron 3_G687T, intron 3_A967G, intron 7_C107T) of 120 individuals from five families of GIFT strain by PCR-RFLP. Correlation analysis between genotypes and weight gain showed that GHR 1 intron 3_A612G and intron 3_A989T associated with male fish weight gain ( P <0.01 and P <0.05) significantly. Similar situation was found in the female population, but the difference was not significant ( P >0.05). Intron 7_A599C was significantly associated with both male ( P <0.01) and female ( P <0.05) fish weight gain. The CC type fish weight gain was significantly higher than AA type’s. On the GHR 2, only intron 3_G687T was found to correlate with female weight gain significantly ( P <0.05). The GT type female fish gain was significantly higher than GG type’s. The correlation between genotypes of 3_A612G, 3_A989T, 7_A599C and 3_G687T and weight gain was detected in 356 individuals from 60 families of GIFT strain (6 individuals in average of each family). There were similar growth situation among different genotypes’ fish, the decreased significance may result in the diverse genetic background and smaller number of samples. Real-time quantitative PCR results indicated that GHR 1 expression in liver of male fish was 1.36 times higher than that in female’s liver, the male fish GHR 2 expression in the tissues (except kidney) was lower than that in the female, which can explain the different effects of GHR 1 and GHR 2 SNP loci on the weight gain of male and female GIFT. In addition, the different influences of GHR1 and GHR2 on the growth result in that only one SNP loci on GHR 2 could affect the weight gain.
引用本文
阮瑞霞,俞菊华,李红霞,李建林,唐永凯,魏可鹏.2011.吉富罗非鱼两种生长激素受体基因的分离及与增重相关的SNPs位点.动物学杂志,46(3):37-46.
复制文章指标
- 点击次数:2332
- 下载次数: 2763
- HTML阅读次数: 0
历史
- 收稿日期:2010-11-15
- 最后修改日期:2011-03-07
