The study determined the filtration rate of Lanceolaria gladiola at different temperatures and pH. The results showed that under the range of 10 to 28℃, the filtration rate of L.gladiola gradually increased with the increasing of temperature, which reached the maximum at 24℃, and then began to decrease. The maximal filtration rate of large, medium and small-sized L.gladiola was 0.826 L/h, 0.768 L/h and 0.653 L/h respectively. At the same temperature,the filtration rates increased with the increasing of the weight of soft part. Temperature, weight of soft part and the interaction between temperature and weight of soft part have significant effect on the filtration rate of L.gladiola ( P ＜0.01). Under different pH grades, the peak value of filtration rate of L.gladiola showed an apparent change. At the pH of 8, the value reached the maximum. The filtration rates for large, medium and small-sized L.gladiola were 0.832 L/h、0.604 L/h、0.421 L/h respectively. When pH was between 6 and 7, the filtration rates for medium and small-sized L.gladiola changed smoothly. Weight of soft part and the interaction between pH and weight of soft part have significant effect on the filtration rate of L.gladiola ( P ＜0.01).