The mating behavior and mating rate of Hirudinaria manillensis were observed under temperature of 24℃, 26℃, 28℃, 30℃, 32℃ and 34℃, both in soil and in water. The hatching number, hatching rate and incubation period were compared among groups with different cocoon weight of <0.6 g, 0.6-1.0 g, 1.0-1.5 g, 1.5-2.0 g and >2.0 g, and under temperatures of 22℃, 24℃, 26℃, 28℃, 30℃ and 32℃, respectively. The results showed that the average mating rate of leeches in soil was significantly higher than that in water, and the highest mating rate occurred in the cases of 28℃ and 30℃ in soil. The cocoon with weights of 1.0-1.5 g and 1.5-2.0 g had the highest hatching number and hatching rate under artificial condition. In addition, temperature influenced hatching significantly, and among 6 temperature groups, the treatments of 26℃ and 28℃ yielded the highest hatching number and hatching rate. With increased temperature, the incubating period of cocoon decreased gradually. The 22℃ group had the longest incubation period of 58 d, while less than 20 d in groups of 30℃ and 32℃.