Total RNAs were extracted from myocardium of Tibetan Antelope (Pantholops hodgsonii) and Tibetan Sheep,both inhabiting on Tibetan Plateau (altitude 4 300 m).PGC-1α coding cDNA sequences were cloned with reverse transcription polymerase chain reaction (RT-PCR),and the sequences were confirmed by DNA sequencing.The cloning and sequencing results confirmed that the PGC-1α gene coding sequences of both Tibetan Antelope and Tibetan sheep showed above 90% identity with other species.In addition,the cloned sequences contained the RNA/DNA binding sites,RRM (RNA recognition motif),the domains involved in the interaction with NRF-1 and MEF2C,Arg/Ser rich domain,negative regulatory domain,LXXLL motif,as well as conserved sequences like TPPTTPP and DHDYCQ,which are present in all PGC-1 family members.Fourteen variable amino acid sites were identified in the functional domains mentioned above.Additionally,analysis of generic phosphorylation sites and kinase specific phosphorylation prediction sites indicated that the 329-threonine amino acid site could be phosphorylated by PKG,which may be unique to Tibetan Antelope.Secondary structures of PGC-1α protein from Tibetan Antelope and Tibetan Sheep were also predicted in this study.In summary,the PGC-1α gene coding regions from Tibetan antelope and Tibetan Sheep have been successfully cloned,which may provide fundamental data for further investigating high altitude adaptation related to genetics in the future.