Polyacrylamide gel electrophoresis was used to detect the expression of ten isozymes (LDH, CCO, EST, CAT, POD, ME, MDH, G6PD, GDH, and ADH) in six different tissues, including eye, gill, skin, muscle, fin, and liver. Part of isozymes gene loci and zymograms were analyzed for providing the foundation for conservation and development of germplasm resources and genetic breeding. The results showed that the electrophoresis patterns of isozymes exhibited an apparent tissue-specificity except for CCO which showed no evident difference in 6 tissues examined. Biochemical genetic analysis showed that the ten isozymes were coded by 21 gene loci, six of which were found to be polymorphic. Finally, based on the zymograms, six isozymes including Est-1, Me-B, s-MDH, G6pd-A, G6pd-B and Adh-A were analyzed genetically for their subunit structure, coding gene loci and numbers as well as their specific expression, and the proportion of polymorphic loci was P=6/21=28.57%.