To study the effects of hypoxia on adrenocorticotropic hormone(ACTH)and blood parameters in Gansu Zokor (Myospalax cansus),we measured the number of red blood cell (RBC),hemoglobin concentration(HGB),hematocrit (HCT),mean corpuscular volume (MCV),mean corpuscular hemoglobin (MCH),and mean corpuscular hemoglobin concentration(MCHC)contents by using Sysmex KX-21N hematology analyzer before and after the hypoxia treatment,and also measured the adrenocorticotropic hormone(ACTH)contents by SN-695A RIA γ measuring instrument before and after hypoxia.The value of RBC,HGB,HCT,and ACTH were significantly higher in the condition that oxygen concentration was 14.1%,10.5%,and 6.5%,while the measurement of MCV did not change significantly,MCH and MCHC decreased after hypoxia adaptation.After intraperitoneal injection of ACTH,the RBC,HGB,and HCT contents all increased evidently.Hypoxia activated hypothalamus-pituitary-adrenal(HPA)and increased ACTH contents in serum,further increased corticosterone(CORT).Those change resulted in the higher value of RBC,HGB,and HCT that would improve the oxygen carrying capacity of blood and adaptation ability of Gansu Zokor to hypoxia.