Illegal trade of Pangolin is becoming more serious, thus forensic identification of criminal material from the molecular level is very urgent and necessary. In order to ascertain whether the processed specimen and scales could be authenticated by DNA molecular technology, total DNA from specimen or scales of Pangolin was extracted with an improved method. Four pairs of primers, which were universal primers for Cyt b gene, primer for complete 12S rRNA sequence, RAPD primer and microsatellite primer were used for amplification. Some amplified fragments were sequenced. The results showed that DNA of almost all these samples could be extracted except for that of the footpad of museum specimen. The extracted DNA could be amplified well with four primers, indicating the value of the present method in species-specific identification and individual recognition study.