In order to provide important scientific basis on the safe use of PVP-I in Carassius auratus gibelio farming, we evaluate the safety of fishery povidonum-iodum(PVP-I)on C.auratus gibelio farming according to the national standards and related regulations. Based on observation on growth inhibition of the PVP-I on Chlorella sp., aquatic probiotic bacteria, we analyzed the acute toxicity of PVP-I to Daphnia magna straus, Brachydanio rerio, and C.auratus gibelio, as well as the influence on main harmful physicochemical factors of the farming water. The results showed that the growth of Chlorella sp. was promoted with PVP-I at the final concentrations of 6.00-14.00 mg/L, its minimum inhibitory concentration to aquatic probiotics were varied from 128 mg/L to 512 mg/L, its 50% inhibitory concentration to Chlorella sp. was estimated to be above 14.00 mg/L, and its 50% lethal dose to D.magna straus, B.rerio were 13.44 mg/L, 17.63 mg/L, respectively. In addition, the 50% lethal dose of PVP-I to C.auratus gibelio was 74.77 mg/L, and in the period of 14 days after the adding of the PVP-I into the farming water at 0.20-1.40 mg/L, the contents of the ammonia and nitrite in C.auratus gibelio farming water were gradually reduced with the increased concentration of the PVP-I. The present study confirmed that the PVP-I had low toxicity, its safe concentration for C.auratus gibelio farming should be no more than 1.34 mg/L in view of its potential threat to zooplankton such as D.magna straus. PVP-I would not lead to the increase of the ammonia and nitrite in the C.auratus gibelio faming water under its safe concentration.