A male and a female of bat Ia io were collected in Dongxuexian Cave (24°58.1' N, 113°11.0' E, 559 m altitude), on 19 September, 2006 and in Wendao Cave(24°56.5' N, 113°6.3' E, 440 m altitude), on 21 November, 2012 respectively,both in Daqiao Town, Ruyuan Country, Guangdong Province. This was the first record for Ia io from Guangdong Province. The feature of morphological structure and skull of the two specimens was given in this paper. At the same time, echolocation calls were record by a Pettersson D-980 bat detector when the bat was flying in spacious indoor conditions, and the acoustic parameters of the male and the female were analyzed respectively. After compared the specimens with those found in Hainan Province and Guizhou Province, it is found that the dominant frequency was obviously lower than others. The specimens were kept in Guangdong Entomological Institute.