White-striped Grass Lizard (Takydromus wolteri) is an oviparous lacertid lizard. Females produce multiple clutches of eggs during the breeding season. To understand the mating system, the paternity of the offspring within and among clutches, the population genetic structure and the evolutionary history of this species, we developed 9 highly polymorphic microsatellites for T. wolteri in a population from Chuzhou, Anhui, China. Loci were isolated from a genomic library enriched for (AC)_n and (ATAG)_n repetitive elements and primers were tested in 16-32 individuals. The number of alleles ranged from 12 to 20 per locus, the expected heterozygosity (H_e) ranged from 0.894 to 0.955, and the observed heterozygosity (H_o) ranged from 0.565 to 0.938. These loci could be useful markers to investigate population genetic structure, gene flow level, population differentiation and mating systems in T. wolteri.