黑琴鸡北方亚种mtDNA D-loop遗传多样性初步研究
作者:
基金项目:

中央高校基本科研业务费专项(No.DL09CA01);国家实验教学示范中心建设专项


Genetic Diversity of the Black Grouse Tetrao tetrix baikalensis at Daxinganling Based on the mtDNA D-loop Sequences
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [25]
  • |
  • 相似文献 [20]
  • |
  • 引证文献
  • | |
  • 文章评论
    摘要:

    黑琴鸡(Tetrao tetrix)为国家Ⅱ级保护动物,但近年来种群数量不断减少。因此,了解不同种群的遗传变异情况,可为制定保护管理策略提供依据,以便恢复野外种群的数量。本文测定加格达奇(42个个体)和呼伦贝尔市扎兰屯(76个个体)两个黑琴鸡种群的共118个个体的mtDNA D-loop 序列,共发现25个变异位点,定义了33个单倍型,整体的平均核苷酸差异数(K)为2.608,核苷酸多样性(π)为0.228%,种群的遗传多样性偏低,两个种群有一定的基因交流,Nm为14.63,群体间无显著的遗传分化。Tajima's D和Fu&Li's D的估算结果表明,这两个黑琴鸡种群相对于中性的歧异度并没有明显的偏离(P>0.1),两个种群可能未经过大规模的种群扩张过程。

    Abstract:

    The Black Grouse (Tetrao tetrix) is one of the class Ⅱnational protected birds in China, and its population is declining in recent years. To understand the difference of genetic variation among populations can provide basic knowledge for the conservation of endangered species, and help to develop management strategies. The genetic diversity of two Black Grouse (T.t.baikalensi) populations at Jagdaqi and Zhalantun, Daxinganling of northeast China were analyzed by the polymorphism of mtDNA D-loop sequences. 118 complete sequences were determined using DNA sequencing technology. 25 polymorphic sites, and 33 haplotypes were defined, of which 4 haplotypes were shared between two populations. The nucleotide diversity(π)of Black Grouse in Jagdaqi and Zhalantun populations were 0.279% and 0.191% respectively, with higher polymorphism in Jagdaqi population. The average number of nucleotide difference (K) and the nucleotide diversity(π)were 2.608 and 0.228% in integral population. There was gene exchange between the two populations(Nm=14.63), and their genetic divergence was not significant. The estimate of Tajima's D and Fu&Li's D didn't deviate significantly from the neutral selection hypothesis(P>0.1)for the two populations, suggesting that they might not have been large-scale population expansion process.

    参考文献
    Anderson S, De Bruijn M H L, Coulson A R, et al. 1982. Complete sequence of bovine mitochondrial DNA conserved features of the mammalian mitochondrial genome. Journal of Molecular Biology, 156(4): 683-717.
    Caigergues A, Rätti O, Helle P, et al. 2003.Population genetic structure of male black grouse (Tetrao tetrix L.) in fragmented vs. continuous landscapes. Molecular Ecology, 12(9): 2297-2305.
    Drovetski S V. 2002. Molecular phylogeny of grouse: individual and combined performance of W-linked, autosomal, and mitochondrial loci. Systematic Biology, 51(6): 930-945.
    Fu Y X. 1997. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 147(2): 915-925.
    Hartl D L, Clark A G. 2006. Principles of Population Genetics. 4th ed. Sunderland: Sinauer Associates, 1-565.
    Höglund J, Larsson J K, Corrales C,et al. 2011. Genetic structure among black grouse in Britain: implications for designing conservation units. Animal Conservation, 14(4): 400-408.
    Höglund J, Larsson J K, Jansman H A H, et al. 2007. Genetic variability in European black grouse (Tetrao tetrix). Conservation Genetics, 8(1): 239-243.
    Neigel J E. 2002. Is FST obsolete? Conservation Genetics, 3(2): 167-173.
    Larsson J K, Jansman H A H, Segelbacher G, et al. 2008. Genetic impoverishment of the black grouse (Tetrao tetrix) population in the Netherlands: detectable only with a reference from the past. Molecular Ecology, 17(8): 1897-1904.
    Librado P, Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11): 1451-1452.
    Rogers A R, Harpending H. 1992. Population growth makes waves in the distribution of pairwise genetic differences. Molecular Biology and Evolution, 9(3): 552-569.
    Slatkin M. 1987. Gene flow and the geographic structure of natural populations. Science, 236(4803): 787-792.
    Tajimaf F. 1989. Statistical method for test the neutral mutation hypothesis by DNA polymorphism. Genetics, 123(3): 585-595.
    Tamura K, Dudley J, Nei M, et al. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8): 1596-1599.
    包文斌, 陈国宏, 吴信生, 等. 2007. 中国红原鸡和泰国红原鸡遗传多样性分析. 遗传, 29(5): 587-592.
    陈晓芳, 李爽, 王黎, 等. 2002. 鸟类线粒体DNA研究概述. 遗传, 24(3): 371-375.
    曹栋. 2011. 河北塞罕坝黑琴鸡的遗传多样性及与栖息地环境因子的相关性研究. 河北: 河北农业大学硕士学位论文, 21-23.
    傅衍, 牛冬, 阮晖, 等. 2001. 浙江省地方鸡种的遗传多样性研究. 遗传学报, 28(7): 606-613.
    贺福银, 王建强, 田家龙, 等.2004. 大兴安岭地区松鸡科鸟类. 中国林副特产, 5(72): 42-43.
    刘铸, 杨春文, 金建丽, 等. 2009. 鸟类线粒体DNA结构特点及在鸟类研究中的应用. 生物技术通报, (12): 42-47.
    马福, 张建龙, 等. 2009. 中国重点陆生野生动物资源调查. 北京: 中国林业出版社, 171-172.
    曲若竹, 侯林, 吕红丽, 等. 2004. 群体遗传结构中的基因流. 遗传, 26(3): 377-382.
    武玉珍, 赵春贵, 王孟本, 等. 2008. 珍稀濒危鸟类褐马鸡mtDNA的分子克隆及序列分析. 山西大学学报: 自然科学版, 31(1): 104-107.
    尹远新, 田家龙. 2009. 我国黑琴鸡种群状况及其保护管理对策. 中国林副特产, (1): 86-87.
    张录强, 李春秋. 1999. 黑琴鸡(Lyrurus tetrix)生物学研究进展(I)——地理分布、亚种分化、食性、生境选择及其行为. 河北师范大学学报: 自然科学版, 23(2): 542-544.
    引证文献
    引证文献 [1]
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

徐秀丽,蔡晓淇,白素英.2013.黑琴鸡北方亚种mtDNA D-loop遗传多样性初步研究.动物学杂志,48(5):673-679.

复制
文章指标
  • 点击次数:2459
  • 下载次数: 2406
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2013-06-08
  • 最后修改日期:2013-08-29
  • 在线发布日期: 2013-10-17