Ex-situ conservation implementation in in-situ conservation is the more effective protection way for endangered wildlife conservation. Since 1976, the Zhalong Nature Reserve has carried out ex-situ conservation works including incubation, training fly and releasement of the Red-crowned Crane (Grus japonensis) based on artificial breeding population. The results showed that from 1976 to now, hatchability of artificial breeding cranes was 77.41% (with the highest of 100% in 1981). Incubation survival number has increased, with the average of 27 individuals per year and the highest of 52 individuals in 2003. In the last decade, the incubation survival number has been kept around 30-50 individuals every year. The scale of training fly program also increased, recently, about 100-120 individuals were trained every year. Associated with the scale of training fly, the scale of releasement was also rising, about 8 individuals was released every year, and after 2009, it was maintained for more than 10 individuals The released Red-crowned Cranes were recorded in the Yancheng Nature Reserve in Jiangsu province, Yellow-river Delta Nature Reserve in Shandong province and Cheorwon near De-military Zone of South Korea. So far, Zhalong Nature Reserve has already got some success.