Based on the field investigational data of Amur Tiger (Panthera tigris altaica) in Heilongjiang Province, we construct the investigate and management Informational system of Amur Tiger in Heilongjiang Province with the combination of the technology of geographic information system, computer technology and the technology of investigation and management about wild animal resources. This system is mainly built by Object-oriented programming language C SHARP, ArcGIS Engine embedded component technology and SQL Server database technology and achieve the graphical and visualized management of Amur Tiger's data in Heilongjiang Province. At the same time, this system is able to provide information on the population quantity the spatial variation timely and accurately by analyzing the spatial survey resources and combining natural factors and human factors in the study area as will as to produce information classification of thematic map and the corresponding field distribution statistical chart according to the analysis results. The system is helpful for the user to manage the survey data and information about wild tiger population and their habitat. It can play an important roll in promoting in situ conservation of wild tiger population and their habitat in China.