During the survey on bat diversity in July and November, 2012, 32 specimens (10 males and 22 females) of Rhinolophus were collected at two caves in Zhijin County of Bijie City and one cave in Wudang District of Guiyang City, Guizhou Province. They were identified as R.paradoxolophus,which is the first record of Rhinolophidae in Guizhou Provinces, The specimens(5 males and 11 females)are preserved in the School of Life Sciences,Guizhou Normal University.