An investigation of commercial fishing was carried out in the middle reaches of the Yangtze River with the purpose to study the status of fish resources in May-June and September-October 2018. Total fish catch was estimated based on the fishery statistic data. The length-structure VPA method was applied to evaluate the stock size of Coreius heteroteron, Parabramis pekinensis and Tachysurus vachelli. The biomass of the firstly five species were 1 077.36 tones, 2 190.74 tones, 58.57 tones, 1 010.54 tones, and 14.55 tones. The total fish biomass for reaches of Yichang City, Shishou City, Honghu City, Wuhan City and Hukou County were 623.25 tones, 698.19 tones, 0.41 tones, 148.65 tones and 0.032 tones (Table 2﹣6), respectively. The results may provide information for assessing protection potentiality and developing fish resource conservation strategy.