In order to understand the resources of amphibians and reptiles in the Shennongjia Dajiuhu Wetland Park, a sparse type of subalpine wetland in China, 12 field surveys were conducted between 2016 and 2018. A total of 16 line-transects (200﹣300 m length) and 24 point-counts (50 m radius) for amphibians, 12 line-transects and 10 point-counts for reptiles were setup for field survey. Questionnaire investigations about amphibians and reptiles were also conducted in 7 local residents. A total of 21 species (2 orders and 8 families) of amphibians were recorded, including 12 species were recorded by field investigation, 2 species were recorded by interview, and 7 species were recorded by references. Seventeen amphibians were oriental realm species, 1 was palaearctic realm species, and 3 were widespread species. Twelve species were listed as Hubei key protected animals; 21 species were listed in the IUCN Red List of Threated Species, including 1 endangered species of Quasipaa boulengeri, and 2 vulnerable species of Liua tsinpaensis and Q. spinosa, and 10 species were endemic to China (Appendix 1). A total of 31 species of reptiles, representing 2 orders and 13 families, were recorded during the period of investigation. Fourteen reptiles were recorded by field investigation, 14 species were recorded by interview, and 3 species were recorded by references. Of them 23 species were oriental realm species, 1 was palaearctic realm species, and 7 were widespread species. Eight species were listed as Hubei key protected animals; 24 species were listed in the IUCN Red List of Threated Species, including 1 endangered species of Mauremys reevesii, and 1 vulnerable species of Naja atra; N, atra was listed in the CITES Appendix II and M. reevesii was listed in the CITES Appendix III; and 12 species were endemic to China (Appendix 2). These results suggest that the amphibians and reptiles in the Shennongjia Dajiuhu Wetland Park are characterized by oriental realm species, endangered species and endemic species to China. This study provides basic data for further research, and for ecological-based conservation and management in biodiversity in the Shennongjia Dajiuhu Wetland Park.