We investigate the infestation and distribution of gamasid mites on Mus pahari based on the field collections in 39 counties of Yunnan Province from 1990 to 2015 as well as the infestations of gamasid mites on M. pahar. We employed patchiness index to analyzed the spatial distribution pattern of gamasid mites and species similarity was analyzed by Jaccard Index. Totally, we collected 14 098 individuals of gamasid mites from the 720 M. pahari mice and identified of 37 species belonging to 12 genera of 2 families. Of them 3 species (Laelaps guizhouensis, L. paucisetosa, L. xingyiensis) were dominant and showed aggregated distributions in each individual of M. pahari. The species and numbers of gamasid mites varied greatly in different landscapes, the infestations (prevalence Pm, mean abundance MA, mean intensity MI) of gamasid mites in mountainous landscape were higher than those in flatland landscape (P < 0.05). The species of gamasid mites found on male and female mice were moderately similar, but males had higher mean abundance and intensity of mites than the female mice did (P < 0.05). The species-plot showed that the gamasid mite species increased as host increase. In conclusion, the infestations of gamasid mites on the body surface of M. pahari were common, and the three dominant mite species were distributed aggregatedly, the composition of gamasid mites on the body surface of M. pahari was different in different landscapes.