Abstract:[Objectives] The nest predation of birds is often affected by nest-site selection. Studying the relationship between nest site and nest predation rate may help to reveal the role of different nest site characteristics in the successful reproduction of birds. [Methods] Using Black-throated Tit (Aegithalos concinnus) nests that were predated and those succeeded in the Dongzhai National Nature Reserve of Henan province from 2014 to 2017 as our study subjects, we investigated the relationship of daily nest survival rate with the found date of nest, and nesting plants by using nest survival analysis with RMark (a R package that provides a formula-based interface for the program MARK). We also assessed their nest-site safety class based on nest-site characteristics and examined whether researchers’ assessment of nest-site safety class could predict daily nest survival rate. Because nest predation might differ between different breeding stages, the egg stage (egg-laying and incubation stage; 124 nests) and nestling stage (119 nests) were analyzed separately. [Results] Daily nest survival rate decreased significantly with increasing nest age at the egg stage (Fig. 1, Table 2), but no significant relationship between nest age and nest survival rate existed in nestling stage (Table 3). In addition, daily nest survival rate did not change with the found dates of the nests and nest safety levels (Table 2, Table 3). However, at the egg stage, the nests built on bamboos (Indocalamus tessellatus, Phyllostachys sp.), Chinese firs (Cunninghamia lanceolate), bushes and grasses (e.g. Rosa spp.) and teas (Camellia sinensis) had a significantly higher daily nest survival rate than those built on cypress (Platycladus orientalis, Juniperus chinensis) and pine trees (Pinus massoniana) (Fig. 2a, Table 2). Furthermore, at the nestling stage, the nests built on bushes and grasses had a significantly lower daily nest survival rate than those built on teas, Chinese firs and bamboos (Fig. 2b, Table 3). [Conclusion] Our results suggest that nesting on certain plants may reduce the nest predation risk of the Black-throated Tit, highlighting the role of nesting plants in influencing the breeding success of birds.