[Objectives] The genus Achalinus is a group of snakes with relatively deficient data. This research provides additional data on this seldom known genus, particularly of the recently described species A. yunkaiensis and the seldom known species A. rufescens. [Methods] The specimen newly reported in this research was identified based on morphological characters and phylogenetic analysis based on cytochrome c oxidase subunit 1 (COI). [Results] This study revealed that a specimen of Achalinus snake, CIB 119041, from Xinning County, Hunan Province was A. yunkaiensis, which was a new provincial record of Hunan (Table 1 & 2; Fig. 1 & 2). The specimen was a female with the following morphological characters:(1) tail length 52 mm, total length 256 mm, tail length/total length 0.203; (2) length of suture between internasals subequal to the suture between prefrontals; (3) supralabials 6, the 4^th﹣5^th touch the eye; (4) infralabials 6, the first 3 touch the first pair of chin shields; (5) ventral scales 150, subcaudal pairs 55, anal entire; (6) temporals 2 + 2 + 3, and the two anterior temporals touch the eye; (7) maxillary teeth 24 (Table 3 & Fig. 3). In addition, a new male topotype of A. rufescens, CIB 119042, was reported from Hongkong with the following characters:(1) tail length 84 mm, total length 418 mm, tail length/total length 0.201; (2) length of suture between internasals longer than the suture between prefrontals; (3) supralabials 6, the 4^th﹣5^th touch the eye; (4) infralabials 5, the first 3 touch the first pair of chin shields; (5) ventral scales 153, subcaudal pairs 62, anal entire; (6) temporals 2 + 2 + 3, and the anterior upper one touch the eye (Table 4, Fig. 4). [Conclusions] This study indicated that A. yunkaiensis represents a new reptile record of Hunan Province, which brought the total numberof Achalinus species in Hunan Province to four. Furthermore, the results revealed that further taxonomic revisions of A. rufescens complex are needed.